Quiz on Analysis and Nucleic Acid Characterization

What is the primary function of restriction enzymes?

• To synthesize DNA

• To cleave DNA at specific sites

• To transfer RNA

• To amplify DNA

What type of sequences do restriction enzymes recognize?

• Linear sequences

• Palindromic sequences

• Circular sequences

• Random sequences

What is the purpose of hybridization in nucleic acid analysis?

• To separate DNA fragments

• To bond complementary DNA or RNA molecules

• To amplify DNA sequences

• To visualize proteins

Which technique is used to evaluate specific DNA sequences?

• Northern blotting

• PCR

• Western blotting

• Southern blotting

What is the main purpose of Southern blotting?

• To amplify RNA

• To identify proteins

• To detect specific DNA sequences

• To analyze RNA sequences

What is the first step in the Southern blot technique?

• Hybridization

• Gel electrophoresis

• DNA digestion

• Probe labeling

What is the role of a probe in hybridization techniques?

• To identify complementary sequences

• To digest DNA

• To amplify DNA

• To separate RNA

Which of the following is a characteristic of Northern blotting?

• It analyzes RNA sequences

• It is used for DNA amplification

• It is used for protein detection

• It focuses on DNA sequences

What is the purpose of the blocking step in blotting techniques?

• To enhance signal detection

• To amplify DNA

• To prevent nonspecific binding

• To digest RNA

What is the main application of Western blotting?

• To amplify nucleic acids

• To analyze DNA sequences

• To visualize RNA

• To detect specific proteins