Nucleic acid hybridization - Southern,Northern hybridization

In which of the following techniques, the DNA is labeled using complementary sequences and then analyzed using autoradiography?

• Western blotting

• Southern blotting

• Isopycnic centrifugation

• Spectrophotometry

The procedure by which DNA gel is transferred to a membrane is termed as ___________________

• blotting

• centifugation

• electrophoresis

• paper chromatography

Which of the following molecules can be analyzed using a northern blot?

• RNA

• Carbohydrates

• Proteins

• DNA

Which of the following is a commonly used label in blotting techniques?

• vimentin

• biotin

• avidin

• streptomycin

During electrophoresis denaturation of the double stranded DNA is brought about by __________

• Treatment with alkali

• Application of current

• Treatment with EtBr

• Application of heat

In case of northern blotting the hybridization occurs between two RNA molecules.

• True

• False

Northern blotting is performed for ____________

• Determining the size of DNA

• Determining the size of RNA

• Quantification of RNA

• Sequencing of RNA

Applications of southern blotting includes

• Identification of transferred genes

• DNA finger printing

• Preparation of RFLP Maps

• All of these

Northern blotting was developed by

• Grunstein and Hogness

• E.M Southern, a British biologist

• James Alwine, George Stark and David Kemp

• Watson & Crick

Which of the following techniques would be most useful to study gene expression?

• Northern blot

• Southern blot

• Western blot

• Eastern blot