SCMOA2 RNA & Protein Tech

What is complementary DNA (cDNA) synthesized from

• DNA

• mRNA

• pre-mRNA

• Introns

Which enzyme is used to synthesize cDNA from mRNA?

• DNA Polymerase

• Reverse polymerase

• RNA Polymerase

• Reverse Transcriptase

Why is cDNA often used in gene expression studies?

• It contains introns

• It is more stable than mRNA

• It is single stranded

• It replicates independently

What is the primary use of a DNA microarray?

• Protein sequencing

• DNA fingerprinting

• Gene expression analysis

• Identifying lipids

What is the role of probes in microarrays?

• Complementary binding with slide

• Complementary binding with fluorescent tags

• Complementary binding with cDNA

What is the main advantage of RNA sequencing over microarrays?

• It is cheaper

• It does not require sequencing

• It provides a comprehensive analysis of gene expression

• It uses radioactivity

Which sequencing technology is most commonly used for RNA-Seq?

• Sanger sequencing

• Illumina

• PacBio

• Nanopore

Which type of RNA is most commonly analyzed in RNA sequencing?

• miRNA

• rRNA

• pre-mRNA

• mRNA

Which technique is commonly used in proteomics to separate proteins based on size?

• PCR

• Agarose electrophoresis

• SDS_PAGE

• Microarrays

Which of the following is a major goal of proteomics?

• Cloning DNA sequences

• Studying lipid synthesis

• Understanding protein interactions and functions

• Identifying RNA modifications