Plasmid Mapping

Where are plasmids located in relation to bacterial chromosomal DNA?

• Inside the chromosomal DNA

• Separate from the chromosomal DNA

• Integrated into the cell wall

• Outside the cell membrane

_________ DNA is created by using the ______ from a bacteria and splicing a section of DNA into it.

• recombinant, plasmid

• Cloning, ribosome

• transcription, translation

• cumulus, mitochondria

How many times do you want a restriction enzyme to cut a human genome in order to cut out the gene of interest to insert into a plasmid?

• 1 - through the gene

• 2 - on either side of the gene

• 3 - through the gene and on either side of the gene

• 4 - twice on each side of the gene

What is a plasmid?

• A small, circular piece of DNA found in bacteria

• A type of protein found in the cell membrane

• A large, linear piece of DNA found in the nucleus

• A type of RNA involved in protein synthesis

Which enzyme is commonly used to cut plasmids for cloning?

• DNA polymerase

• Ligase

• Restriction enzyme

• Helicase

What is the purpose of plasmid mapping?

• To determine the sequence of nucleotides in a plasmid

• To identify the location of genes and restriction sites on a plasmid

• To measure the size of a plasmid

• To replicate plasmids in bacterial cells

In plasmid mapping, what is the role of gel electrophoresis?

• To amplify DNA fragments

• To separate DNA fragments by size

• To sequence DNA

• To cut DNA at specific sites

Which of the following is a common feature of plasmids used in genetic engineering?

• They contain an origin of replication

• They are always linear

• They cannot be transferred between cells

• They are resistant to all antibiotics

How can plasmids be introduced into bacterial cells?

• Through transformation

• By binary fission

• Via mitosis

• Through meiosis

What is a selectable marker in the context of plasmid mapping?

• A gene that allows for the identification of cells that have taken up the plasmid

• A sequence that initiates DNA replication

• A site where DNA is cut by restriction enzymes

• A protein that binds to DNA