DNA Sequencing

What is the purpose of Polymerase Chain Reaction (PCR) in the process of sequencing DNA?

• To separate DNA fragments by size

• To synthesize copies of template DNA

• To change the structure of template DNA

• To terminate the elongation of a DNA strand

Which part of Sanger (Cycle) Sequencing is NOT found in regular Polymerase Chain Reaction (PCR)?

• DNA polymerase

• Primers

• Deoxyribonucleotides (dNTPs)

• Dideoxyribonucleotides (ddNTPs)

• DNA template

How are deoxyribonucleotides different from dideoxyribonucleotides? Choose all that apply.

• Deoxyribonucleotides are used in both PCR and Cycle Sequencing.

• Dideoxyribonucleotides are missing an OH group compared to deoxyribonucleotides.

• You cannot add new nucleotides to a deoxyribonucleotide.

• Deoxyribonucleotides are NOT found in natural living cells.

• Dideoxyribonucleotides are labeled with fluorescent tags.

Tags

NGSS.HS-LS1-1

What do dideoxynucleotides do when added to a growing DNA strand during Cycle Sequencing?

• They stop the elongation of that strand.

• That double the length of that strand.

• They shorten the length of the strand.

• They denature the strand.

What is the primary purpose of DNA sequencing?

• To replicate DNA

• To determine the order of nucleotides in DNA

• To repair DNA

• To express genes

Which part(s) of a hair can be analyzed for DNA?

• root

• cuticle

• medulla

• cortex

This is the last step of PCR and it requires the temperature to increase to 72C for one minute. Here, complementary strands will be produced from the corresponding base pairs, reforming the DNA into two new strands.

• Elongation by DNA Polymerase

• Primer Annealing

• Denaturation

What is the correct letter sequence that matches this DNA?

This is the last step of PCR and it requires the temperature to increase to 72C for one minute. Here, complementary strands will be produced from the corresponding base pairs, reforming the DNA into two new strands.

• Elongation by DNA Polymerase

• Primer Annealing

• Denaturation

Match the DNA sequence TAC with the matching DNA sequence.

• ATG

• TAC

• UTG

• AUG