PCR Quiz

The first step in PCR is

• Primer annealing

• Primer extension

• Denaturation

• None of these

What is the purpose of PCR?

• To produce millions of copies of DNA.

• To produce millions of copies of a specific region of DNA

• To add nucleotides to a DNA sequence

• To watch polymerase work.

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PCR

Topic 2C1

The PCR technique was developed by

• Karry Mullis

• Milstein

• Kohler

• Altman

What is the denaturation step?

• mutating RNA polymerase

• separating DNA helix in a cold environment

• Cooling DNA to allow primers to form

• separating DNA double helix with using heat

At what temperature does the Denature step of PCR occur?

• 94-96° C

• 72° C

• 50-65° C

• Any temperature

What happens during the annealing step of PCR?

• Primers are created.

• Primers copy the new DNA strand.

• The primers attach to the target DNA region.

• Primers sequence DNA

What is the extension step?

• DNA polymerase adds nucleotides to the 5' end

• DNA polymerase adds nucleotides to the 3' end

• RNA polymerase adds nucleotides to the 5' end

• RNA polymerase adds nucleotides to the 3' end

Why does PCR require a specific type of polymerase?

• Taq polymerase can withstand the range of temperatures in PCR without denaturing.

• Taq polymerase is the most common type of polymerase.

• Taq polymerase is the most inexpensive type of polymerase.

• A specific type of polymerase is unnecessary.

Which of the following statements are true regarding PCR

• Automated PCR machines are called thermal cyclers

• Billion copies of desired DNA can be synthesized from microgram quantities of DNA

• A thermostable DNA Polymerase is required

• All of these are ture

PCR is a

• DNA Sequencing technique

• DNA Degradation technique

• DNA Amplification technique

• All of these