Mi

During PCR, what happens during the Denaturation step?

• DNA strands are separated according to size using gel electrophoresis.

• Primers form base pairs with the single-stranded template DNA.

• DNA polymerase adds new nucleotides to the primer to elongate the new strand.

• Hydrogen bonds between bases are broken using high heat to separate DNA strands.

During which PCR step do primers form complementary base pairs with single-stranded template DNA?

• Denaturation

• Annealing

• Extension

• Synthesis

Which part of Sanger (Cycle) Sequencing is NOT found in regular Polymerase Chain Reaction (PCR)?

• DNA polymerase

• Primers

• Deoxyribonucleotides (dNTPs)

• Dideoxyribonucleotides (ddNTPs)

• DNA template

How are deoxyribonucleotides different from dideoxyribonucleotides? Choose all that apply.

• Deoxyribonucleotides are used in both PCR and Cycle Sequencing.

• Dideoxyribonucleotides are missing an OH group compared to deoxyribonucleotides.

• You cannot add new nucleotides to a deoxyribonucleotide.

• Deoxyribonucleotides are NOT found in natural living cells.

• Dideoxyribonucleotides are labeled with fluorescent tags.

Tags

NGSS.HS-LS1-1

Which letter represents amplitude? (a)  

• A

• B

• C

• D

• E

Tags

NGSS.HS-PS4-1

How is DNA synthesis in PCR and Cycle Sequencing the SAME?

• Both require DNA polymerase.

• Both require primers.

• Both require deoxynucleotides (dNTPs).

• Both require dideoxynucleotides (dNTPs).

• Both generate new DNA products of equal length.

DNA has a ___________ charge and will migrate through a gel towards the ___________ electrode.

• positive, negative

• positive, positive

• negative, positive

• negative, negative

Larger DNA fragments move through a gel _____________ smaller DNA fragments.

• faster than

• at the same rate as

• slower than

What do dideoxynucleotides do when added to a growing DNA strand during Cycle Sequencing?

• They stop the elongation of that strand.

• That double the length of that strand.

• They shorten the length of the strand.

• They denature the strand.

Which of the following base pairs is matched correctly?

• Guanine and Thymine

• Cytosine and Adenine

• Adenine and Thymine

• Thymine and Cytosine