Manipulating DNA Biology

This cuts DNA at specific sequences

• Plasmid
• Recombinant DNA
• Restriction enzyme
• Gel electrophoresis

This diagram represents which of the following? 

• Gel electrophoresis
• Gene therapy
• Cloning 
• Genetic engineering

How do restriction enzymes "know" where to cut DNA

• A specific sequence of nucleotides

• They cut randomly

• Distance from the centromere

"Sticky-ends" are DNA strands with:

• single-stranded ends

• ends cut flat by an enzyme

• no way to be recombined

Which is used to cut DNA molecules at specific points?

• gel electrophoresis
• PCR analysis
• a restriction enzyme
• recombinant DNA

Enzyme that connects the DNA to the backbone (glues)

• Ligase

• Polymerase

• Helicase

• Primase

DNA polymerase moves in the _________ to ____________ direction

• 3' to 5'

• easiest

• 5" to 3"

• 9 to 5

DNA strand has the following bases AAGCCA what are the bases on the complementary strand

• GGCTTA

• TTGGGA

• CCAGGT

• TTCGGT

Number 1 in the picture above

• Replication

• DNA Polymerase

• Primase

• Ligase

Once the gene and plasmid have been cut with a restriction enzyme, another enzyme called _________ is used to join the pieces of DNA together.

• polymerase
• ligase
• dehydrogenase
• lactase