PCR and Gel Electrophoresis

What is the purpose of PCR?

• To produce millions of copies of DNA.
• To produce millions of copies of a specific region of DNA
• To add nucleotides to a DNA sequence
• To watch polymerase work.

At what temperature does the Denature step of PCR occur?

• 94-96° C
• 50-65° C
• 72° C
• Any temperature

Why does PCR require a specific type of polymerase?

• Taq polymerase can withstand the range of temperatures in PCR without denaturing.
• Taq polymerase is the most inexpensive type of polymerase.
• Taq polymerase is the most common type of polymerase.
• A specific type of polymerase is unnecessary.

The first step in PCR is

• Primer annealing

• Denaturation

• Primer extension

• None of these

What happens during the Anneal step of PCR?

• Primers are created.
• The primers attach to the target DNA region.
• Primers copy the new DNA strand.
• Primers sequence DNA

What is the denaturation step?

• mutating RNA polymerase

• Cooling DNA to allow primers to form

• separating DNA helix in a cold environment

• separating DNA helix with using heat

What is the Extension step?

• DNA polymerase adds nucleotides to the 5' end

• RNA polymerase adds nucleotides to the 5' end

• DNA polymerase adds nucleotides to the 3' end

• RNA polymerase adds nucleotides to the 3' end

Which of the following statements are true regarding PCR

• Automated PCR machines are called thermal cyclers

• A thermostable DNA Polymerase is required

• Billion copies of desired DNA can be synthesized from microgram quantities of DNA

• All of these

the basic requirements of PCR reaction include

• DNA segment to be amplified

• Two oligonucleotide primers

• a heat stable DNA polymerase

• all of these

Reverse transcription PCR uses

• mRNA as a template to form

• RNA as a template to form a DNA

• DNA as a template to form ssDNA

• all of these