Transcription

Name the enzyme that facilitates opening of DNA helix during transcription.

• DNA polymerase

• RNA polymerase

• DNA ligase

• DNA helicase

Which of the following is a wrong match?

• Rho factor - Terminates translation

• Template strand – Has same sequence as of mRNA

• Sigma factor - Initiates translation

• All of these

Code in RNA corresponding to ATGC in DNA

• TACG

• TACA

• AGUC

• UACG

In the absence of which factor or enzyme, the transcription starts non-specifically (hypothetically) at any base of DNA ?

• Rho ( ρ ) factor

• Core enzyme

• Sigma ( σ ) factor

• None of the above

In initiation of transcription, there is

• Attachment of RNA polymerase holoenzyme at promoter site.

• Terminal addition of 200-300 adenylated nucleotide at 3' end of m-RNA

• ATPase activity by rho factor resulting in release of polynucleotide chain

• Binding of multiple ribosomes at single mRNA.

Which of the following sequences of RNA will be transcribed from a hypothetical sequence of DNA given below?

3'-ATGCATGCATGCATGC-5'

5'-TACGTACGTACGTACG-3'

• 3'-UACGUACGUACGUACG-5'

• 5'-AUGCAUGCAUGCAUGC-3'

• 5'-UACGUACGUACGUACG-3'

• 3'-AUGCAUGCAUGCAUGC-3'

Find the correct statement regarding eukaryotic transcription

• There is single DNA dependent RNA polymerase that catalyses transcription of all types of RNA

• Elongation is controlled by sigma factor

• Termination factor associates with DNA polymerase enzyme during termination of transcription

• RNA polymerase II catalyses the transcription of hnRNA in eukaryotes.

Mark the option depicting correct labelling for the image given below.

• A- Template strand, B- Coding Strand, C- Terminator, D-Promoter

• A- Structural gene, B- Template strand, C- Promoter, D- Terminator

• A- Coding Strand, B- Template strand, C- Terminator, D- Promoter

• A- Coding Strand, B- Structural gene, C- Promoter, D- Terminator

Promoter region is located towards the

• 5’ end of the coding strand of gene

• 3’ end of the template strand of gene

• 5’ end of the template strand of gene

• Both (A) and (B)

If the sequence of coding strand is 5’ GCCGTACCGTTACG 3’ , then the sequence of mRNA will be

• 5’ GCAUUGCCAUGCCG 3’

• 3’ CGGUACCGUUAGCC 5’

• 5’ GCCGUACCGUUACG 3’

• 3’ CGGCAUGGCAAUGC 5’