tut26quiz

Eye colour 1 and 2 are on the same gene, but eye colour 1 and 3 are not on the same gene. Further, if eye colour 3 and 5 are non complementary, how many genes are required for eye colour synthesis?

• 1
• 2
• 3
• 4
• 5

Epistasis involved the intersection between how many genes?

• 2
• 3
• 4
• 5
• 6

When determining the A-P axis, what needs to happen before homeotic selector gene expression to determine what specific segments will become ex. specific body parts such as the leg, arm, body?

• a. Genes need to be labelled and organised in the cell
• b. The Pair-rule genes must be expressed
• c. Homeotic selector genes are used to create a zygotic gap
• d. Regulation requires no hierarchy and genes are pre dispositioned to become a specific part
• e. Multiple cleavage cycles determine which segments will be cleaved into a body part

Which of the following methods of inserting exogenous DNA into yeast involves a targeted method?

• Injection of exogenous DNA directly into yeast cells.
• Transposon-mediated transformation to insert exogenous DNA into the genome.
• Homologous recombination with DNA fragments including the gene of interest.
• Inserting plasmids containing random fragments of the wild-type genome.
• Use episomes with selectable markers to interest exogenous DNA into the yeast.

You have cloned a gene using functional complementation. The gene MARS25 is required for transcription of DNA and to activate a transcription factor. In the null mars25 loss of function allele, transcription does not occur and the transcription factor is not activated. You have made 4 mutations in the coding region of MARS25 in the plasmid carrying MARS25 that is able to complement the mars25 allele when functional. The first mutant complements/rescues both transcription and activation of the transcription factor. The second mutant rescues transcription and activates the transcription factor, but causes it to be ON all the time. The third mutant does not rescue transcription or activation of the transcription factor. The fourth mutant rescues transcription but not activation of the transcription factor.How would you classify these alleles (in the order 1-4)?

• A) functional allele, dominant negative allele, loss of function allele, haploinsufficient allele
• B) functional allele, gain of function allele, loss of function allele, hypomorphic allele
• C) gain of function allele, dominant negative allele, loss of function allele, conditional allele
• D) dominant allele, gain of function allele, loss of function allele, hypomorphic allele
• E) gain of function allele, loss of function allele, functional allele, hypomorphic allele

What is true for both siRNA and miRNA?

• Both transcribe an inverted repeat, which forms a hairpin structure
• Both create a RISC complex (RNA induced silencing complex)
• Both prevent translation using RISC
• Both start with double stranded RNA
• None of these are shared

In a genetic cross between two organisms, where one gene suppresses the effect of another gene, this interaction is an example of:

• A) Synthesis
• B) Epistasis
• C) Pleiotropy
• D) Polygenic inheritance
• E) None of the above

Which of the following methods involves the alteration of gene activity by introducing small RNA molecules that specifically target mRNA for degradation or translational inhibition?

• Forward genetics
• Reverse genetics
• CRISPR
• siRNA
• miRNA

Suppose alleles X and Y complement each other and are NOT found in the same gene, what does that say about their complementation?

• Complementation and they're in different complementation groups
• Non-complementation and they're in different complementation groups
• Complementation and they're in the same complementation group
• Non-complementation and they're in the same complementation group
• None of the above

Why can two independent mutations on different alleles in the leucine gene, involved in leucine synthesis, lead to a leu (+) organism?

• The mutations occur in regulatory regions upstream of the leu gene, enhancing transcriptional activity.
• One mutation results in a loss-of-function mutation in an enzyme involved in leucine synthesis, while the other mutation results in a gain-of-function mutation.
• The mutations occur in separate genes, so that each diploid gene has a functional copy that can compensate for the deficiencies of the other copy.
• Both mutations lead to changes in the tertiary structure of the leu protein, forming a functional enzyme complex.
• None of the above.