Sequencing 08 Nov 2023

Shotgun sequencing method is called a chemical sequencing method.

• TRUE

• FALSE

In Maxam Gilbert method, the chemical used for identifying cytosine and thymine is….

• Dimethyl sulphate in formic acid + piperidine

• Dimethyl sulphate + piperidine

• Hydrazine + piperidine

• Hydrazine in NaCl + piperidine

In the automated (modern) DNA sequencing, radio labelled ddNTPs are used.

• TRUE

• FALSE

What will dimethyl sulphate methylate in normal environment?

• Cytosine

• Guanine

• Purine

• Pyrimidine

Bioinformatics does not requires complete genome sequences to be useful.

• TRUE

• FALSE

What is the specific format used to copy our sequence into BLAST?

• NGS

• MEGA

• SEQ

• FASTA

Starting from the sequencing primer, what is the sequence of this DNA sample? (write the sequence direction!)

What are the types of web BLAST? (more than one answers is possible)

• nucleotide BLAST

• blastn

• blastx

• protein BLAST

• primer BLAST

What is the main process that happen in a sequencing machine?

• ELISA

• SDS-PAGE

• PCR

• Electrophoresis

• Colorimetric

What is the program’s name that we used for editing DNA sequence in this sequencing practicum?

• NCBI

• BLAST

• Chromas

• BioEdit