Lecture 1 Quiz (16S rRNA gene sequence analysis)

What is the function of 16S rRNA in bacterial cells?

• To encode genes for antibiotic resistance

• To form the cell membrane

• To act as a template for protein synthesis

• To serve as a marker for bacterial ID

Which of the following is not a step in the 16S rRNA sequence analysis pipeline?

• DNA Extraction

• PCR Amplification

• Mass Spectometry

• Sequence Alignment

What is the primary advantage of using Staden for 16S rRNA analysis?

• It is free and open-source

• It can handle large datasets efficiently

• It has a user-friendly interface

• It can perform phylogenetic analysis

What is reference database in 16S rRNA sequence analysis?

• A tool for editing and analyzing sequence data

• A set of previously sequenced and annotated 16S rRNA sequences used for comparison

• A type of PCR primer used for amplifying specific regions of the 16S rRNA gene

• A method for identifying chimeric sequences in a dataset

What is the disadvantage of using only a single 16S rRNA variable region for bacterial identification?

• It can lead to false positive identifications

• It can result in low sequence coverage or quality

• Cause high percent identity

• It can miss important taxonomic information

What is the size range of the 16S rRNA gene in bacteria?

• 1,500 bp

• 1,200 bp

• 1,350 bp

• 1,000 bp

Which variable region(s) of the 16S rRNA gene is/are commonly used for bacterial identification?

• V1-V2

• V1-V3

• V4-V5

• V1-V9

What is the percent identify threshold commonly used for bacterial identification using 16S rRNA sequencing?

• 95%

• 98%

• 99%

• 100%

What is the purpose of generating a consensus sequence from bidirectional reads in 16S rRNA sequencing?

• To improve the accuracy and quality of the sequence

• To create multiple copies of the gene

• To produce another sequence

• To reduce the length of the sequence

What is the main limitation of using 16S rRNA sequencing for bacterial identification?

• It is not a ubiquitous gene

• Give the genus level

• It may not differentiate between closely related species

• It will not provide taxonomic classification