Sequencing 07 Nov 2023

What causes the termination of chain elongation in DNA sequencing?

• The incorporation of DNA nucleotide

• The incorporation of dideoxynucleotide

• The denaturation of the double-stranded fragments

• The reaction of dimethyl sulfate and piperidine

Which of the following statements is incorrect regarding Sanger sequencing method?

• Using chemical

• Determined by DNA synthesis of complementary polynucleotide chains

• There is the termination of chain elongation at specific nucleotide positions

• Using enzyme

In Shotgun sequencing method, where do we store our DNA fragments?

• Cell Membrane

• Nucleoid

• Plasmid

• Nucleus

DMS methylate guanine in acidic environment.

• TRUE

• FALSE

What is the sequence of the DNA fragment from the chromatogram?

What are sequence methods? (more than one answer is possible)

• Next generation sequencing

• Sanger sequencing

• Cycle sequencing

• Chemical degradation sequencing

• Advanced sequencing

No hydroxyl group at 3’ end of dNTP prevents strands extension.

• TRUE

• FALSE

As in PCR method, a pair of primer is required in Sanger method DNA sequencing.

• TRUE

• FALSE

Original Sanger method uses ³²P-deoxynucleoside triphosphates in primer labeling.

• TRUE

• FALSE

After we have a sequence data, we can identify the sequences that are similar to nucleotide sequences in a sequences database to the software named BLAST.

• TRUE

• FALSE