Biochemical Test

A colony of bacteria is placed over a drop of 3% hydrofen peroxide solution immediate oxygen bubbling is observed? What do you say about the bubbling colony of bacterial?

• Catalase +ve
• Catalase -ve
• Lactose fermenting
• Gram -ve

In the slide method of the coagulase test, clumping of the organisms is seen within 10 seconds after adding human plasma to one of the suspensions. What is the interpretation?

• Coagulase negative
• Coagulase positive
• Coagulase neutral
• Result not clear

At what temperature and duration should the test tube be incubated in the test tube method of coagulase test?

• 40-47° C for 6 hours
• 25-30° C for 3 to 4 hours
• 35-37°Cfor10-12 hours
• 35-37°C for3to 4 hours

Which of the following test methods are used in the oxidase test procedure?

• Filter paper method
• Direct plate method
• Swab method
• All of the method

In the oxidase test, after adding the reagent how long do we need to wait to see the color changes?

• 10 sec
• 10 min
• 1 hrs
• 1 days

If we are seeing deep purple-blue color after adding reagent in the oxidase test what does that mean?

• Positive test
• Negative test
• Neutral test
• No interpretation

Which of the following reagent is NOT used in the oxidase test?

• Gordon and McLeod's Reagent
• Kovacs Oxidase Reagent
• Bromothymol Reagent
• Gaby and Hadley (indophenol oxidase) reagent

Which of the following reagent is used in the direct plate method of oxidase test? 1. Kovac's oxidase reagent 2. Covid reagent 3. Gordon and McLeod reagent 4. Jordon and McConkey reagent

• 1 and 3
• 1,3and 4
• 1 and 4
• 2 and 3

In what type of agar plate the direct plate method of oxidase test is carried out?

• Non-selective agar plate [+]
• Selective agar plate
• Heating plate
• Nutrient plates

What is ‘A’ in the following sentence? When using Gordan and Mcleod reagent, microorganisms are oxidased positive whenthe colour changes to "A"

• Red within 10 -30 min
• Black within 60 minutes
• Both A & B
• All of the above