Practical-1, Enzyme Purifcation

What is the source of enzyme?

• Acid Phosphatase

• Alkaline phosphatase

• Moong bean sprouts

• Liver tissue

What is the enzyme you are purifying?

• Acid phosphatase

• Alkaline phosphatase

• Phosphohexose isomerase

• Glycogen phosphorylase

What is the sequence of purification?

• grinding-filtering--dialysis-centrifugation-precipitation

• centrifugation-grinding-precipitation-dialysis-filtering

• grinding-filtering-centrifugation-precipitation-dialysis

• dialysis-grinding-centrifugation-precipitation-filtering

What salt is used salt fractionation in your experiment?

• Aluminum sulphate

• Ammonium Sulphate

• Sodium chloride

• Sodium sulphate

how long one should keep the sample for dialysis normally

• 1 hr

• 10 hr

• 24 hrs

• 48 hrs

This is the chief principle of Dialysis

• Capillary action

• Adhesion

• Osmosis

• Cohesion

What is the composition of the membrane that is used in Dialysis?

• Chitin

• Polyethylene

• Polyvinyl chloride

• Cellulose

Proteins are stored in

• Sodium Azide

• At ultra low temperature with cryoprotectant

• In lyophilized form

• All of the above

What is homogenization

• Removing the cell debris

• Centrifugating the samples

• Making a homogenous mixture of sample and buffer

• None of the above

What is the benefit of partial purification?

• Less steps are required

• Less time consuming

• Cost effective

• All of the above