L4&5 protein structure and enzymes

What method would you use to separate a protein based on charge

• Electrophoresis

• Gel Filtration

• pI precipitation

• Ammonium sulfate precipitation

What level of protein structure requires more than 1 polypeptide chain.

• Primary

• Secondary

• Tertiary

• Quaternary

What of the following are secondary structures

• Active sites

• Alpha helixs

• Beta sheets

• Gamma coils

Select all the factors that could cause protein denaturation

• Heat

• Organic solvents

• pH extremes

• Detergents

Which of these is not a function of an Enzyme

• Store molecules

• Bind to substrates

• Catalyze reactions

• Transfer organic groups

How do Enzymes speed up reactions.

• Enzymes provide a higher activation energy for reactions meaning more reactants can react

• Enzymes provide a lower activation energy for reactions meaning more reactants can react

• Enzymes alter the charge of reactants making them more likely to react

• Enzymes heat up reactants giving them more energy and making them more likely to react

What effect does heat on biological reactions

• Increases the reaction velocity

• Decreases the reaction velocity

• Increases the reaction velocity to a point, then the reaction rate will plateau

• Increases the reaction velocity to a point, then the reaction rate will plummet.

What do Ligase Enzymes do

• Re-arrange functional groups

• Join molecules together

• Break bonds

• Move functional groups

What is the enzyme model called where, the enzyme changes its shape to form an enzyme substrate complex, then reverts to its original shape.

• Lock and Key Model

• Induced Fit Model

Enzymes act as regulators for the production of organic molecules but enzymes are not actually regulated themselves as there naturally exist the correct amount of enzymes in the cell at all times.

• True

• False