Technique in DNA Technology 2.0

1. 1. What temperature is used in PCR to denature the DNA strands?

• 37°C
  

• 42°C

• 72°C

• 94°C

2. What enzyme catalyzes the amplification of DNA during PCR?

• Ligase

• Polymerase

• Telomerase

• Primase

3. What is the main advantage of producing transgenic Bt corn?

• Improved nutritional quality

• Enhanced cold tolerance

• Built-in insect resistance

• Increased crop yield

How are most GMOs generated?

• Selective breeding

• Chemical mutagenesis

• Recombinant DNA technology

• Hybridization

5. Which of these sequencing methods relies on dideoxynucleotides to terminate DNA synthesis?

• Maxam-Gilbert sequencing

• Next-generation sequencing

• Sanger sequencing

• Nanophore sequencing

7. How does recombinant DNA differ from natural DNA?

• It contains introns

• It is synthesized chemically

• It brings together DNA from different sources

• It has methylated bases

8. What is the purpose of a restriction enzyme in DNA cloning?

• To ligate DNA fragments

• To amplify cloned DNA

• To cut DNA at specific sites

• To denature double-stranded DNA

9. PCR can be used in which of these fields?

• Agriculture

• Nutrition

• Forensic science

• All of the above

What happens in the Denature step of PCR?

• The DNA nucleotides are broken apart.
• The base-pairing rules for DNA are reversed.
• The double-stranded DNA is separated into two single strands of DNA.
• The DNA is returned to its natural setting.

What is the function of a primer?

• To identify the particular region of DNA to be copied by PCR.
• To copy DNA.
• To create DNA nucleotides.
• To maintain the temperature of the PCR reaction.