Biotechnology Semester 1 Final Practice

Front

What does PCR stand for?

Back

Polymerase Chain Reaction

Front

What is the complementary DNA sequence for AATCAGC?

Back

TTAGTCG

Front

Which suspect's DNA matches the blood stain DNA in a gel electrophoresis?

Back

Suspect 3

Front

Which STR allele has the greatest DNA fragment length in an electropherogram?

Back

FGA

Front

When running DNA samples through a gel, where are they loaded and where do they move towards?

Back

Loaded in wells on the negative side; they move toward the positive side.

Front

What is the role of DNA polymerase in DNA replication?

Back

DNA polymerase synthesizes the new DNA strand by adding complementary nucleotides.

Front

What is gel electrophoresis used for?

Back

To separate DNA fragments based on their size.

Front

Define STR (Short Tandem Repeat).

Back

A short sequence of DNA that is repeated multiple times in a row.

Front

What is the purpose of primers in PCR?

Back

Primers are short sequences of nucleotides that provide a starting point for DNA synthesis.

Front

What is the significance of the negative and positive electrodes in gel electrophoresis?

Back

DNA is negatively charged and moves towards the positive electrode.