Neutral Buffered Formalin Quiz

What is the primary reason for adding a phosphate buffer to formalin?

• To make it more volatile

• To enhance its color

• To stabilize its pH to neutral

• To increase its acidity

Why is neutral buffered formalin preferred over ordinary formalin for histological studies?

• It is more readily available

• It has a stronger odor

• It prevents acid hematin formation

• It is cheaper

What is a notable characteristic of specimens preserved in ordinary formalin for decades?

• They disintegrate quickly

• They lose color

• They maintain structural integrity

• They become toxic

Under which condition is it acceptable to use ordinary unbuffered formalin?

• When the specimen is for scientific research

• When the specimen has a calcareous exoskeleton

• When buffered formalin is unavailable

• When the specimen is aquatic

What type of skin makes a specimen more suitable for preservation in ordinary formalin?

• Colorful skin

• Transparent skin

• Thick and resistant skin

• Thin and fragile skin

What is the first step in preparing 10% Neutral Buffered Formalin?

• Pour water into a beaker

• Weigh 4 grams of monobasic phosphate

• Stir the solution

• Measure 100 ml of formaldehyde

Which type of water is NOT recommended for preparing NBF?

• Salt water

• Tap water

• Distilled water

• Rain water

What is the purpose of using a weighing scale in the preparation of NBF?

• To check the temperature

• To determine the pH level

• To measure the volume of water

• To weigh the phosphate salts accurately

What formula is used to calculate the dilution of NBF?

• E=mc^2

• N1V1 = N2V2

• PV=nRT

• F=ma

What is the final step in preparing a diluted NBF solution?

• Top up with water

• Heat the solution

• Stir the solution

• Add more phosphate