
Understanding qPCR Techniques

Interactive Video
•
Biology
•
10th - 12th Grade
•
Hard
Nancy Jackson
FREE Resource
5 questions
Show all answers
1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the primary purpose of qPCR in the context of viral infections?
To sequence the entire genome of a virus
To quantitatively determine the presence of a target sequence or gene
To amplify DNA sequences for genetic research
To create vaccines for viral infections
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
How is the progression of a qPCR reaction monitored?
By measuring the temperature changes
By counting the number of cycles manually
By visualizing the accumulation of the amplification product with fluorescent reporters
By observing color changes in the solution
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What does a low Ct value indicate in a qPCR analysis?
DNA degradation
Low DNA concentration
High DNA concentration
No DNA present
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
In the Cyber Green assay, what role does the intercalating dye play?
It acts as a quencher for the fluorescent signal
It binds to single-stranded RNA
It initiates the denaturation process
It binds to double-stranded DNA
5.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is a key difference between the Cyber Green and TaqMan qPCR methods?
Cyber Green requires a higher temperature for denaturation
TaqMan uses a probe that is cleaved to emit fluorescence, while Cyber Green uses an intercalating dye
TaqMan is only used for bacterial DNA
Cyber Green uses a probe, while TaqMan uses an intercalating dye
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