Gene Technology Quiz

What is the term used for swapping genes between different species?

• Gene cloning

• Gene sequencing

• Gene therapy

• Genetic modification

What is the first step in the process of genetic modification?

• Using DNA ligase

• Transforming the host genome

• Inserting the gene into a vector

• Isolating the desired gene

What is the role of restriction endonucleases in gene technology?

• To cut DNA at specific sequences

• To replicate RNA

• To synthesize new DNA strands

• To bind DNA fragments together

What is a potential issue when using restriction endonucleases?

• They cannot cut DNA at all

• They can cut DNA at unintended sites

• They only work on RNA

• They require high temperatures to function

What does reverse transcriptase do in the process of gene isolation?

• Replicates DNA

• Converts DNA into RNA

• Cuts DNA at specific sites

• Converts RNA into DNA

Why is using mRNA and reverse transcriptase advantageous in gene isolation?

• It requires more steps

• It avoids introns in the gene

• It provides incomplete genes

• It is more expensive

What is the final step in creating a double-stranded cDNA?

• Using DNA ligase

• Using RNA polymerase

• Using restriction enzymes

• Using DNA polymerase

What enzyme is used to join sticky ends together in genetic modification?

• DNA polymerase

• RNA polymerase

• DNA ligase

• Reverse transcriptase

What type of bonds does DNA ligase form to join DNA fragments?

• Hydrogen bonds

• Covalent phosphodiester bonds

• Ionic bonds

• Peptide bonds

What is the purpose of inserting a gene into a vector?

• To replicate the gene

• To transport the gene into the host

• To destroy the gene

• To sequence the gene