Lichen Chemical Spot Testing Techniques

Sarah Norvell demonstrates how to identify lichens using chemical spot testing. She explains the setup, identifies the genus Hypogymnia, and discusses the K, C, and P tests. The tutorial includes a practical demonstration of these tests, highlighting the importance of color changes in identifying lichen species. The video concludes with the identification of Hypogymnia heterophylla.

What is the primary purpose of Sarah Norvell's video tutorial?

• To demonstrate how to use a microscope

• To show how to identify lichens using chemical spot testing

• To explain the history of lichens

• To discuss the ecology of lichens

Which resource does Sarah recommend for learning more about lichen chemical spot testing?

• The Lichenologist's Handbook

• Lichens of North America

• McCune and Geiser's Macrolichens of the Pacific Northwest

• The Complete Guide to Lichens

What is the K-test solution made of?

• 95% Ethanol

• P-Phenylenediamine

• 5% Sodium Hypochlorite

• 10% Potassium Hydroxide

Which chemical is used in the C-test?

• P-Phenylenediamine

• Ethanol

• Sodium Hypochlorite

• Potassium Hydroxide

What precaution should be taken when using the P-test?

• It should be kept in the dark as it is light-sensitive

• It should be used with gloves only

• It should be refrigerated

• It should be mixed with water

What color change indicates a positive K-test on the lichen cortex?

• Yellow

• Blue

• Orange

• Red

What is the expected result of the K-test on the medulla?

• Blue color change

• Red color change

• Yellow color change

• No color change

What combination of tests is used to see an orange color change?

• K-test and C-test

• P-test and K-test

• C-test and P-test

• K-test and P-test

What does a yellow to red color change in the P-test indicate?

• No lichen present

• Incorrect test application

• Hypogymnia heterophylla

• Hypogymnia inactiva

What should be done with the lichen sample after testing?

• Send it to a laboratory

• Preserve it for future use

• Dispose of it properly

• Store it in a dark place