Leukocyte Count Experiment Concepts

Anish Kulkarni, a first-year MBBS student, demonstrates the total leukocyte count experiment. He explains the necessary apparatus, preparation steps, and procedure, including using a pipette and Nubus chamber. The video covers microscope usage for counting white blood cells, calculations, and the dilution factor. It also addresses questions on leukocyte count, causes of leukocytosis, and WBC production. The video concludes with potential sources of error and their minimization.

What is the primary purpose of using Turk's fluid in the leukocyte count experiment?

• To sterilize the equipment

• To dilute the blood sample

• To stain the white blood cells

• To increase the blood volume

Why is the ring finger preferred for pricking during the experiment?

• It is easier to clean

• It provides more blood

• It is not connected to the bursa

• It is less painful

What is the total volume of the Neubauer chamber used in the experiment?

• 0.1 mm³

• 0.4 mm³

• 1.0 mm³

• 0.5 mm³

How is the dilution factor of 20 achieved in the experiment?

• By adding 1 ml of blood to 11 ml of diluting fluid

• By adding 0.5 ml of blood to 20 ml of diluting fluid

• By adding 1 ml of blood to 20 ml of diluting fluid

• By adding 0.5 ml of blood to 11 ml of diluting fluid

What is the normal range of white blood cells in the human body?

• 1,000 to 4,000

• 20,000 to 50,000

• 4,000 to 11,000

• 11,000 to 20,000

Which component of Turk's fluid stains the nuclei of white blood cells?

• Eosin

• Distilled water

• Methyl violet

• Glacial acetic acid

What is leukocytosis?

• Decrease in white blood cells

• Decrease in red blood cells

• Increase in white blood cells

• Increase in platelets

Which of the following is a physiological cause of leukocytosis?

• Typhoid fever

• Pregnancy

• Leukemia

• Irradiation

Where are white blood cells produced in the body?

• Liver

• Spleen

• Bone marrow

• Lymph nodes

What is a potential source of error if the pipette is wet?

• It may cause the bead to stick

• It may dilute the blood sample

• It may lead to inaccurate blood volume

• It may cause air bubbles