Producing Monoclonal Antibodies: From Lymphocytes to Hybridomas

The video tutorial explains how antibodies are produced by lymphocytes and their role in binding to specific antigens. It introduces monoclonal antibodies, which are identical antibodies produced from a single cell clone, and discusses their applications in targeting proteins. The tutorial also covers the challenges in producing monoclonal antibodies due to lymphocytes' inability to divide, and how hybridoma technology, which fuses lymphocytes with tumor cells, overcomes this issue. The process of producing monoclonal antibodies, including the use of mice and hybridoma cells, is detailed.

What are antibodies and how are they produced?

Explain the concept of monoclonal antibodies.

What are the advantages of using monoclonal antibodies in targeting specific cells?

What is a hybridoma cell and how is it formed?

Describe the process of producing monoclonal antibodies.