Michaelis-Menten Plot: Determine 4 Kinetic Parameters (Part 1)

The video tutorial explains the Michaelis-Menten curve and how to estimate four kinetic parameters: VMAX, KM, Kcat, and catalytic efficiency. It discusses the limitations of the Michaelis-Menten method and introduces the more accurate Lineweaver-Burk plot. The tutorial provides step-by-step instructions for calculating each parameter and highlights the importance of using correct units.

How can you determine the substrate concentration at half of the VMAX?

What are the units for Kcat and how are they derived?

What does catalytic efficiency indicate in enzyme kinetics?

Why is the Lineweaver-Burk plot considered more accurate than the Michaelis-Menten plot?