DNA Extraction Quiz

What is the main purpose of lysis buffer in DNA extraction?

• Neutralize DNA

• Break open cell membranes

• Precipitate proteins

• Denature DNA

Why is salt (NaCl) added to the lysis buffer?

• To make the DNA taste better

• To help DNA clump together by neutralizing its charge

• To kill bacteria in the sample

• To change the color of the solution

What component of the cell must be broken to release DNA?

• Nucleus only

• Mitochondria

• Cell wall/membrane

• Ribosomes

Cold alcohol is used in DNA extraction to:

• Dissolve DNA

• Deactivate enzymes

• Precipitate DNA

• Remove lipids

Which alcohol concentration is BEST for spooling long DNA strands?

• 50% ethanol

• 70% ethanol

• 96% ethanol

• 100% ethanol

what does the white/transparent, stringy substance in the alcohol layer represent?

• Dissolved proteins

• Clumped DNA molecules

• Salt crystals

• Soap bubbles

Why is DNA extraction important in pharmacogenomics?

• To reduce drug cost

• To diagnose diabetes

• To analyze genetic drug response

• To make vaccines