GMO Plants and Reporter Genes Quiz

What is the primary purpose of the screening process in GMO plants?

• To measure the expression of native genes

• To identify successfully transformed plants

• To monitor plant growth rates

• To study environmental stress effects on plants

Which of the following reporter gene systems is constitutive and drives high expression in all tissues?

• GFP

• Luciferase

• Anthocyanin

• GUS

What is the function of cis-acting elements in promoters?

• To bind to transcription factors and regulate gene expression

• To translocate proteins across membranes

• To provide substrates for reporter gene assays

• To stabilize mRNA during transcription termination

Why is the GUS reporter system frequently used in plant studies?

• It is stress-inducible and responds to environmental changes.

• It produces a blue color upon reaction, which is easy to detect.

• It relies on bioluminescence for sensitive gene monitoring.

• It is a natural part of plant genomes.

What does 'temporal expression' of a protein refer to?

• The location of protein production within tissues

• The activation of proteins by light stimuli

• Changes in protein levels over time in response to biological cues

• The production of proteins under environmental stress

What is the main role of the SAG21 promoter in plants?

• To drive high expression in response to temperature changes

• To regulate gene activity under biotic or abiotic stress

• To bind cis-elements for light response regulation

• To promote auxin synthesis during growth

Which factor influences protein spatial expression?

• Subcellular localization

• Developmental regulation

• Stimulus-responsive expression

• Time-specific synthesis

What does the acronym GUS stand for in the GUS reporter system?

• Glucuronidase Unique System

• β-Glucuronidase

• Genetic Utility Sensor

• Gene Upregulation System

What does a colorless result indicate in a GUS assay?

• Positive expression of the target gene

• Negative expression of the target gene

• Stress-induced promoter activity

• Temporal changes in gene expression

Which promoter component serves as a molecular switch to regulate gene activity?

• Trans-acting factors

• Reporter genes

• Cis-acting regulatory elements

• Restriction sites