DNA Replication Quiz

What is the nature of DNA replication?

• Conservative

• Semi-conservative

• Dispersive

• Non-conservative

Which enzyme unwinds and separates the two DNA strands during replication?

• DNA Polymerase

• Ligase

• Helicase

• Primase

What is the role of DNA polymerase in DNA replication?

• Unwinds DNA strands

• Joins free nucleotides to form a complementary strand

• Breaks hydrogen bonds between base pairs

• Synthesizes RNA primers

What is the average length of a chromosome in base pairs?

• 100 million bp

• 150 million bp

• 200 million bp

• 250 million bp

How fast does DNA polymerase copy DNA?

• ~10 bp/sec

• ~30 bp/sec

• ~50 bp/sec

• ~70 bp/sec

What is the role of Taq polymerase in the polymerase chain reaction (PCR)?

• It separates DNA strands.

• It binds primers to DNA.

• It copies the DNA strands.

• It fragments the DNA.

At what temperature does the denaturation step occur in PCR?

• 55°C

• 72°C

• 90°C

• 37°C

What is the purpose of gel electrophoresis in DNA analysis?

• To amplify DNA sequences.

• To separate DNA based on size.

• To bind primers to DNA.

• To denature DNA strands.

What type of gel is used for DNA samples in gel electrophoresis?

• Polyacrylamide gel

• Agarose gel

• SDS gel

• Starch gel

What are short tandem repeats (STR) used for in DNA profiling?

• To amplify DNA sequences.

• To identify and compare individuals.

• To denature DNA strands.

• To bind primers to DNA.