BioinfoUI Module 1 Quiz

The term "bench" in "bench to server" primarily refers to:

• A computational server

• A public database

• The laboratory space and activities

• An assembly software tool

The process of breaking open cells to release DNA is called:

• DNA ligation

• DNA quantification

• DNA extraction

• DNA sequencing

DNA isolation or purification refers to:

• Washing the cells with alcohol

• Amplifying the DNA

• Removing contaminants like proteins and lipids from extracted DNA

• Sequencing purified DNA

The first visible sign that DNA extraction worked in a test tube is often:

• Green fluorescence

• Bubbles

• A white, thread-like precipitate

• A shift in pH

Before sequencing, DNA is quantified to determine:

• Its species

• GC content

• Concentration in ng/μL

• Ribosomal contamination

Which tool gives accurate quantification of double-stranded DNA?

• NanoDrop

• Gel electrophoresis

• Qubit

• PCR

DNA normalization is performed to:

• Improve GC content

• Equalize input concentrations before pooling

• Adjust base pair ratios

• Reduce sequencing bias from long reads

Barcoding/indexing enables:

• Sequencing longer DNA fragments

• Color detection

• Multiplexing different samples in one sequencing run

• Increasing fragment size

The number of DNA samples that can be pooled in one sequencing run depends on:

• Genome size and number of barcodes

• Extraction temperature

• Magnesium concentration

• GC percentage

Choosing a single colony is essential for:

• Getting more DNA

• Ensuring purity of the sequence data

• Improving read length

• Speeding up sequencing