PASS W10 Content

Which site of the ribosome holds the tRNA carrying the growing polypeptide chain?

• E site

• P site

• A site

• T site

Which of the following mutations is least likely to affect protein function?

• Frameshift mutation

• Missense mutation

• Silent mutation

• Nonsense mutation

Which mutation is most likely to produce a nonfunctional protein?

• Silent mutation

• Missense mutation

• Nonsense mutation

• Conservative missense mutation

Which process uses restriction enzymes, plasmids, and bacteria for DNA manipulation?

• PCR

• Recombinant DNA technology

• Gel electrophoresis

Which sequence initiates translation in prokaryotes?

• 5' cap

• AUG codon

• Shine–Dalgarno sequence

• Poly-A tail

What happens when a stop codon enters the A site?

• A tRNA brings an amino acid

• The ribosome shifts

• A release factor binds

• Translation restarts

Which DNA molecule would migrate more slowly through an agarose gel during electrophoresis?

• The shorter DNA molecule

• The longer DNA molecule

• The more negatively charged DNA molecule

• The circular DNA molecule

Which of the following steps in PCR is responsible for determining the specificity of the PCR reaction?

• Denaturation

• Extension

• Replication

• Annealing of primers to the DNA template

Which of the following best describes the process of Sanger sequencing?

• Separating DNA fragments by size using a gel

• Amplifying a specific region of DNA using PCR

• Inserting a gene into a plasmid vector

• Identifying the specific order of nucleotides in a DNA fragment

Which of the following statements best describes the role of restriction enzymes in genetic engineering?

• They unwind the DNA double helix

• They cut DNA at specific recognition sequences

• They replicate DNA

• They ligate DNA fragments together