Biochem Ch 7 : Nucleic Acid

What is recombinant DNA (rDNA)?

• DNA that is naturally occurring in a single organism.
• A type of RNA used in protein synthesis.
• 2 different DNA that has not been altered or modified by technology.

• DNA that has been artificially created by combining 2 or more DNA sequences through gene splicing.

Which of the following is NOT the step of recombinant DNA?

• Isolating

• Ligation

• Adding

• Cutting

Choose the correct sequence of steps of making rDNA.

• Cutting → Isolating → Transforming → Joining → Cloning → Screening

• Isolating → Cutting → Joining → Transforming → Cloning → Screening

• Isolating → Cloning → Joining → Transforming → Cutting → Screening

• Screening → Cloning → Transforming → Joining → Cutting → Isolating

Restriction enzyme can cut DNA into either...

(Multiple answer)

• blunt end

• sharp end

• smooth end

• sticky end

Which is true about plasmids?

• Plasmids can be cut at specific sequences called restriction sites.
• Plasmids contain a promoter sequence that defines where transcription begins.
• Plasmids can contain an antibiotic resistance gene.
• All of the statements are true about plasmids.

Where do we insert vectors?

• Bacterial host cells

• Eestriction enzymes

• Culture media

• DNA

Which of the following are the correct steps in mismatch repair mechanism of single stranded DNA?

• Incorrect nucleotide incorporated

• Excision of incorrect nucleotide

• Addition of correct nucleotide

• Separation of incorrect nucleotide using restriction enzyme

In DNA replication, the structure created after splitting starts is known as...

• Starting Point

• Replication Point

• Replication Fork

• Replication Spoon

Which enzyme that removes RNA primers in DNA replication?

• DNA Polymerase III

• DNA Polymerase I

• DNA Polymerase II

• RNA Polymerase

The leading strand is read using..

• 3'-5' direction

• 5'-3' direction

• 5'-6' direction

• 6'-5' direction