Quiz on Chromatography Techniques

What is the advantage of lining the inside of the jar with filter paper?

• It yields straight solvent fronts

• Developing time is reduced to one third

• RF values are much less than in unsaturated tanks

What is the process of recovery of constituents from the chromatogram known as?

• Elution

• Development

• Adsorption

What is the purpose of the optical detectors in chromatography?

• To determine the dissolved substances

• To introduce the mixture into the column

• To affect the process of development

What is the main requirement for adsorbents used in chromatography?

• Particles should be spherical in shape

• They should react chemically with the eluting solvent

• They should contain a large amount of soluble components

What is the function of solvents in chromatography?

• To introduce the mixture into the column

• To affect the process of development

• To remove the required content of each zone

What is the advantage of gradient development in chromatography?

• It allows for continuous change in solvent composition

• It is useful for separating compounds of similar properties

• It reduces the time required for development

What is the purpose of the flame ionisation detectors?

• To pyrolyse organic components

• To measure the temperature of the flame

• To detect the presence of ions in the flame

What is the role of the stationary phase in chromatography?

• To provide a medium for the separation of components

• To act as a solvent for the mobile phase

• To increase the temperature of the system

Which factor is crucial for optimizing the resolution in chromatography?

• Flow rate of the mobile phase

• Temperature of the stationary phase

• Volume of the sample injected

What is the significance of retention time in chromatography?

• It indicates the time a compound spends in the stationary phase

• It affects the color of the eluted compounds

• It determines the temperature of the mobile phase