Gene Expression & Biotechnology

Bacterial mating

• is mediated by bacteriophage

• involves transfer of DNA between living cells

• involves direct uptake of DNA from the environment

• results in a higher frequency of genetic change than can be accounted for by mutation due to duplication errors

• does not require cell-to-cell contact

Transformation

• is mediated by bacteriophage

• involves transfer of DNA between living cells

• involves direct uptake of DNA from the environment

• results in a higher frequency of genetic change than can be accounted for by mutation due to duplication errors

• does not require cell-to-cell contact

Transduction

• is mediated by bacteriophage

• involves transfer of DNA between living cells

• involves direct uptake of DNA from the environment

• results in a higher frequency of genetic change than can be accounted for by mutation due to duplication errors

• does not require cell-to-cell contact

produces a collection of identical cells

• palindrome

• cloning

• vector

• chimera

a genetic hybrid; contains DNA from different sources

• recombinant DNA

• chimera

• vector

• palindrome

plasmid or virus used to introduce genes into a host cell

• restriction endonuclease

• vector

• cloning

• recombinant DNA

DNA duplex with antiparallel sequence

• palindrome

• restriction endonuclease

• cloning

• chimera

DNA that contains a desired inserted gene

• recombinant DNA

• vector

• chimera

• cloning

recognizes/cleaves DNA sequence

• restriction endonuclease

• cloning

• chimera

• vector

separation of charged molecules

• denaturation

• annealing primers

• primer extension

• DNA fingerprint

• gel electrophoresis