Unit 1 Quiz DNA

Q1. The helical turn of B-DNA contains approximately how many base pairs?

• 5
• 10
• 15
• 20

Q2. What is the role of Chloroform in organic extraction of DNA?

• Protein solubility
• RNA stability
• Phase separation
• Cell lysis

Q3. Which buffer is commonly used in agarose gel electrophoresis for DNA?

• PBS
• TAE or TBE buffer
• Glycine buffer
• Citrate buffer

Q4. Which method is used after gel electrophoresis to transfer DNA onto a membrane for further analysis?

• Northern blotting
• Southern blotting
• Western blotting
• Eastern blotting

Q5. During DNA extraction, which solution helps to dissolve lipids?

• SDS
• Isopropanol
• Ethanol
• Tris buffer

Q6. The A260/A280 ratio is used to assess

• DNA purity
• DNA size
• DNA sequence
• DNA melting point

Q7. Which spectrophotometer is commonly used for "micro-volume" DNA quantification?

• Mass spectrometer
• Beckman counter
• HPLC
• Nanodrop

Q8. In slot blot, the sample is applied directly to:

• Agarose gel
• Nitrocellulose or nylon membrane
• SDS-PAGE gel
• ELISA plate

Q9. Hybridization in slot blot involves:

• PCR primers
• A labeled complementary probe
• DNA polymerase
• RNA polymerase

Q10. The mitochondrial genome is best described as:

• Linear and double-stranded
• Circular and double-stranded
• Circular and single-stranded
• Fragmented and linear