Protein Assay

Quantification of protein using a UV spectrophotometer is based on ____

• UV light is absorbed by the backbone of the protein

• UV light is absorbed by the peptide bonds of the protein

• UV light is absorbed by the aromatic amino acids of the protein

• UV light is absorbed by all the non-aromatic amino acids of the protein

Biuret assay is based on ____ interaction with protein

• Cu2+

• Brillant blue

• Folin-Ciocalteu reagent

Bradford Assay is based on ____ interaction with protein

• Cu2+

• Coomassie Blue

• Folin-Ciocalteu reagent

Which amino acids does Coomassie dye mainly interact with in the Bradford assay?

• Arginine, Tryptophan, Tyrosine

• Histidine, Cysteine, Arginine

• Arginine, Tryptophan, Histidine

• Tryptophan, Tyrosine, Cysteine

What is the Bradford Assay?

• A cooking technique for vegetables

• A method for measuring blood pressure
• A colorimetric protein assay

• A type of dance

What is the purpose of creating a standard curve?

• It allows you to determine the concentration of an unknown solution.

• It allows you to estimate the absorbance of a substance with a known concentration.

• It provides you with the entire absorption spectrum of a substance.

• It allows you to determine what chemical elements are present in a solution.