Protein Assay

Protein Assay

University

6 Qs

quiz-placeholder

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Protein Assay

Protein Assay

Assessment

Quiz

Biology

University

Medium

Created by

Rouzbeh Chegeni

Used 1+ times

FREE Resource

6 questions

Show all answers

1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Quantification of protein using a UV spectrophotometer is based on ____

UV light is absorbed by the backbone of the protein

UV light is absorbed by the peptide bonds of the protein

UV light is absorbed by the aromatic amino acids of the protein

UV light is absorbed by all the non-aromatic amino acids of the protein

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Biuret assay is based on ____ interaction with protein

Cu2+

Brillant blue

Folin-Ciocalteu reagent

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Bradford Assay is based on ____ interaction with protein

Cu2+

Coomassie Blue

Folin-Ciocalteu reagent

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which amino acids does Coomassie dye mainly interact with in the Bradford assay?

Arginine, Tryptophan, Tyrosine

Histidine, Cysteine, Arginine

Arginine, Tryptophan, Histidine

Tryptophan, Tyrosine, Cysteine

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the Bradford Assay?

A cooking technique for vegetables

A method for measuring blood pressure

A colorimetric protein assay

A type of dance

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the purpose of creating a standard curve?

It allows you to determine the concentration of an unknown solution.

It allows you to estimate the absorbance of a substance with a known concentration.

It provides you with the entire absorption spectrum of a substance.

It allows you to determine what chemical elements are present in a solution.