Quantification of protein using a UV spectrophotometer is based on ____

Protein Assay

Quiz
•
Biology
•
University
•
Medium

Rouzbeh Chegeni
Used 1+ times
FREE Resource
6 questions
Show all answers
1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
UV light is absorbed by the backbone of the protein
UV light is absorbed by the peptide bonds of the protein
UV light is absorbed by the aromatic amino acids of the protein
UV light is absorbed by all the non-aromatic amino acids of the protein
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Biuret assay is based on ____ interaction with protein
Cu2+
Brillant blue
Folin-Ciocalteu reagent
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Bradford Assay is based on ____ interaction with protein
Cu2+
Coomassie Blue
Folin-Ciocalteu reagent
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which amino acids does Coomassie dye mainly interact with in the Bradford assay?
Arginine, Tryptophan, Tyrosine
Histidine, Cysteine, Arginine
Arginine, Tryptophan, Histidine
Tryptophan, Tyrosine, Cysteine
5.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the Bradford Assay?
A cooking technique for vegetables
A colorimetric protein assay
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the purpose of creating a standard curve?
It allows you to determine the concentration of an unknown solution.
It allows you to estimate the absorbance of a substance with a known concentration.
It provides you with the entire absorption spectrum of a substance.
It allows you to determine what chemical elements are present in a solution.
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