MIDTERM LAB EXAM - AU

What is the primary purpose of electrophoresis?

• To synthesize proteins

• To analyze the movement of molecules

• To amplify DNA

• To purify nucleic acids

Which gel type is commonly used for the separation of larger DNA fragments?

• Polyacrylamide Gel

• Capillary Gel

• Agarose Gel

• Cellulose Gel

What is the role of buffer systems in electrophoresis?

• To carry the current and protect samples

• To provide nutrients to the samples

• To increase the temperature of the gel

• To enhance the visibility of DNA

Which dye is most widely used for DNA visualization in electrophoresis?

• Coomassie Blue

• Ethidium Bromide

• Fluorescein

• SyBr Green

What is the purpose of using a tracking dye during electrophoresis?

• To monitor the progress of the electrophoresis

• To increase the pH of the buffer

• To enhance the resolution of the gel

• To stain the DNA

What does the term 'reptation' refer to in electrophoresis?

• The separation of proteins

• The application of electric current

• The process of gel polymerization

• The movement of DNA through the gel

Which of the following is a characteristic of polyacrylamide gels?

• They cannot be altered for pore size

• They are derived from seaweed

• They have consistent resolution characteristics

• They are used only for protein analysis

What is the significance of the OD260/OD280 ratio in DNA purity assessment?

• It indicates the concentration of RNA

• It assesses the gel's pH level

• It helps determine the presence of proteins

• It measures the size of DNA fragments

Which buffer is known for having a greater buffering capacity but is prone to precipitation?

• Tris acetate EDTA (TAE)

• Tris borate EDTA (TBE)

• Tris phosphate EDTA (TPE)

• Sodium phosphate buffer

What is the main advantage of capillary electrophoresis over traditional slab gel electrophoresis?

• Lower cost

• Increased sensitivity

• Easier sample preparation

• Longer analysis time