Recombinant DNA Quiz

What is the first step in making recombinant DNA?

• Cloning

• Screening

• Isolating the gene of interest

• Transformation

Which enzyme is used to isolate the gene of interest?

• DNA ligase

• Restriction enzyme

• Polymerase

• Helicase

How many times should the DNA be cut when isolating the gene of interest?

• Once

• Twice

• Three times

• Four times

Where should the DNA not be cut when isolating the gene of interest?

• At the start

• In the middle

• At the end

• In the self-replicating gene

What is removed from bacteria to make recombinant DNA?

• Chromosome

• Plasmid

• Nucleus

• Ribosome

What is used to cut the plasmid from bacteria?

• Different restriction enzyme

• Same restriction enzyme

• DNA polymerase

• RNA polymerase

What joins the DNA and plasmid to make recombinant DNA?

• DNA polymerase

• RNA ligase

• DNA ligase

• Helicase

What is the vector in making recombinant DNA?

• Chromosome

• Plasmid

• Nucleus

• Ribosome

What is added to another test tube with bacterial cells during transformation?

• Recombinant plasmid

• Chromosome

• Nucleus

• Ribosome

What is the purpose of cloning in bacterial transformation?

• To destroy bacterial cells

• To allow bacterial cells to reproduce

• To isolate the gene of interest

• To cut the plasmid