GeneticsLab - Gel Electrophoresis

Sarah and Jacob performed a gel electrophoresis experiment. The results are represented in the diagram below. Compared to the fragments at the top of the gel, the fragments at the lower end are:

• larger, and move slower

• larger, and move faster

• smaller, and move faster

• smaller, and move slower

Based on these results, whose blood was found in the blood stain at the crime scene?

• Ruby

• Sue

• John

• Lisa

Austin and Sarah are working on a science project to analyze DNA samples. What technique should they use to separate DNA based on size?

• helps cut DNA

• counts the genes in DNA

• separates DNA based on size

• allows for an exact replicated organism

During a science class experiment, DeAngelo and Gabrielle are observing the movement of DNA fragments in gel electrophoresis. Why do the fragments of DNA travel away from the negative electrode?

• DNA is negatively charged, so it is attracted to the positive end of the unit

• DNA is positively charged, so it is attracted to the negative end of the unit

• The agarose gel is negatively charged

• The agarose gel is positively charged

Which male is the father of this child?

• Male 1

• Male 2

• Neither could be the father

Ruby and Wyatt are conducting a gel electrophoresis experiment in their biology class. They want to know where the largest DNA fragment will appear on the gel. What should they expect?

• closest to the starting wells

• farthest from the starting wells

• three quarters away from the starting wells

• it depends on how many fragments there are

John and Gabrielle are setting up an electrophoresis experiment in their biology class. Help them put the steps of setting up the electrophoresis apparatus in the correct order:

1. ​ ​ (a)   ​

2. ​ (b)  

3.​ (c)  

4. ​ (d)  

5. ​ (e)  

• Prepare the gel

• Load the samples

• Run the electrophoresis

• Stain the gel

During a science class experiment, Gabrielle and Jacob are running a gel electrophoresis to separate DNA fragments. Which of the following describes the orientation of the DNA found in their run gel electrophoresis?

• Small fragments are found near the well since it is negatively charged.

• Large fragments are found near the well while small fragments are found at the other end.

• Small fragments are found near the well while small fragments are found at the other end.

• Large fragments are found near the well since it is positively charged.

During a biology lab, Hien and Austin are performing gel electrophoresis to analyze DNA samples. Which of the following explains why some bands are darker in their gel?

• This is a location where there is a secondary well in the gel.

• There are a large number of fragments of the same size.

• The DNA at that band absorbed more dye since it is more positively charged.

• This is an error in the gel electrophoresis caused by a clogged pore in the gel.

During a science experiment, Dezah and Wyatt were tasked with moving DNA samples down a gel. What provides the electrical current needed for this process?

• Power Source

• Lane

• Well

• Tank

• Marker / DNA Ladder