BIOTECHNOLOGY: PRINCIPLES AND PROCESSES

Which of the following is not a feature of plasmids?

• Extra chromosomal

• Circular structure

• Single stranded

• Independent replication

The procedure through which a piece of DNA is introduced into a host bacterium is

• Amplification

• Cloning

• Transformation

• Restriction

Autonomously replicating circular extra-chromosomal DNA is called

• Vector

• Restriction enzyme

• Marker

• Plasmid

Plant cells are bombarded with high velocity microparticles of gold or Tungsten coated with DNA in a method known as

• Microinjection

• Biolistics

• Heat shock

The commonly used vector for cloning genes in animals is

• Rhino viruses

• Disarmed retrovirus

• Disarmed Ti plasmid

• Vector mediated

• Agrobacterium tumifaciens

Enzymes which remove nucleotides from the ends of the D.N.A. are

• Endonucleases

• Exonucleases

• DNA Ligase

• DNA polymerase

Restriction site of EcoR1 enzyme is

• 5'-GAATTC-3'

• 3'-GAATTC-5'

• GAATTC

• 5'-CTTAAG-3'

The technique used to separate DNA in recombinant DNA technology is

• Gel electrophoresis

• PCR

• Autoradiography

• Downstream processing

Which of the following is the stain used to visualize DNA fragments in gel electrophoresis?

• Cotton blue

• Ethidium bromide

• Acetocarmine

• Saffranine

The extraction of separated bands of DNA by cutting out them from the agarose gel is

• Denaturation

• Fragmentation

• Elution