MI Unit 4 Cram Sheet Quiz

What is the primary focus of Lesson 4.1 in the Unit 4 Cram Sheet?

• Manufacturing human proteins

• Studying the effects of diabetes

• Learning about cow insulin

• Exploring plant genetics

Why was human insulin preferred over cow insulin?

• It was cheaper to produce

• It was more effective and safer

• It was easier to extract

• It had fewer side effects

What organism is used to produce human insulin in Lesson 4.1?

• Plants

• Bacteria

• Animals

• Fungi

What is the role of plasmids in bacterial transformation?

• They act as a nutrient source

• They replicate the bacteria

• They contain genes for human proteins

• They destroy unwanted DNA

What is the purpose of using lysozyme in the protein purification process?

• To bind proteins in solution

• To rupture cell membranes

• To separate hydrophobic proteins

• To add a binding buffer

What is the role of the chromatography column in the protein purification process?

• To grow bacterial colonies

• To incubate bacteria at 37°C

• To separate proteins based on their properties

• To centrifuge the bacteria

Why is a low-salt buffer added to the chromatography column?

• To bind proteins in solution

• To collect bacterial colonies

• To separate hydrophobic proteins

• To rupture cell membranes

What is the first step in the process of transforming bacterial colonies with pGLO plasmid DNA?

• Inoculate into nutrient broth containing ampicillin and arabinose

• Pick a single fluorescent green colony from the agar plate

• Grow overnight at 32°C

• Add high-salt chromatography binding buffer

On Day 2, what is added to the bacterial lysate to help bind GFP to the chromatography matrix?

• Low-salt buffer

• Medium-salt buffer

• High-salt chromatography binding buffer

• Lysozyme

What is the purpose of adding lysozyme and freezing the cells on Day 2?

• To bind GFP to the chromatography matrix

• To wash bacterial proteins from the column

• To rupture cell membranes

• To inoculate into nutrient broth