High Performance Liquid Chromatography (HPLC) Quiz

What is the primary function of the pump in HPLC?

• To inject the sample

• To maintain stable flow of the mobile phase

• To detect the analytes

• To filter the mobile phase

Which type of chromatography is most widely used in liquid chromatography?

• Size Exclusion Chromatography

• Partition Chromatography

• Reversed Phase Chromatography

• Ion Exchange Chromatography

What is the purpose of a guard column in HPLC?

• To enhance the sensitivity of the detector

• To inject samples automatically

• To prevent clogging of the analytical column

• To increase the pressure in the system

Which of the following is NOT a type of detector used in HPLC?

• UV Absorbance

• Refractive Index

• Mass-Spectrometric

• Gas Chromatography

What is the main advantage of using HPLC?

• It is only suitable for volatile compounds

• It provides greater sensitivity

• It is less expensive than other methods

• It requires large sample sizes

What does the term 'isocratic elution' refer to?

• Using a constant solvent composition

• Using multiple columns in series

• Eluting with gas instead of liquid

• Changing the solvent composition continuously

What is the role of the degasser in HPLC?

• To filter out solid particles

• To remove dissolved gases from the liquid

• To increase the temperature of the mobile phase

• To inject samples into the system

In reversed-phase chromatography, which component elutes first?

• The smallest molecule

• The most polar component

• The least polar component

• The largest molecule

What type of packing is used in size exclusion chromatography?

• Silica gel only

• Non-porous glass beads

• Metallic particles

• Porous microparticles

Which of the following is a method to degas the solvent?

• Filtration

• Centrifugation

• Ultrasound

• Heating