Chapter 3 - DNA Sequencing - 1 to 25

What does DNA sequencing determine?
a) The shape of the DNA molecule
b) The order of nucleotides in DNA
c) The length of the DNA molecule
d) The number of cells in DNA

• a

• b

• c

• d

Which of the following is a characteristic of Sanger sequencing?
a) It uses RNA instead of DNA
b) It is faster than all other sequencing methods
c) It involves synthesizing DNA templates and terminating synthesis with modified nucleotides
d) It does not require primers for DNA synthesis

• a

• b

• c

• d

What is the role of the primase enzyme in Sanger sequencing?
a) To stop the DNA synthesis
b) To add modified nucleotides to the sequence
c) To start the DNA synthesis process
d) To cleave the DNA into fragments

• a

• b

• c

• d

In Sanger sequencing, what happens if the DNA polymerase picks a modified nucleotide (e.g., A')?
a) The synthesis continues
b) The DNA sequence is incomplete
c) The synthesis stops
d) The DNA is replicated

• a

• b

• c

• d

In Sanger sequencing, the DNA polymerase always picks the normal nucleotide to continue synthesis.

• True

• False

Sanger sequencing requires a specific enzyme called "primase" to start the synthesis process.

• True

• False

What is the Maxam-Gilbert sequencing method used for?
a) DNA replication
b) Breaking DNA at specific nucleotides using chemical treatments
c) Amplifying DNA
d) Synthesizing new DNA strands

• a

• b

• c

• d

Which chemical is used to break DNA at adenine (A) or guanine (G)?
a) Hydrazine
b) Dimethyl sulfate
c) Sodium chloride
d) Formic acid

• a

• b

• c

• d

Why is Maxam-Gilbert sequencing less commonly used today?
a) It requires a special enzyme
b) It is faster than newer methods
c) It is less efficient and more complex than newer sequencing methods
d) It only works on small DNA samples

• a

• b

• c

• d

Dimethyl sulfate is used to break DNA at cytosine (C).

• True

• False