Plasmid Mapping

These show the length of DNA fragments between restriction sites in a strand of DNA.

• restriction maps

• cloning

• gene sequencing

• bioinformatics

What do we call the small, circular pieces of DNA inside a bacterial cell?

Bacterial DNA that is often circular or in the shape of a ring is called a:

• Clone

• Plasmid

• Fragment

• Recombinant DNA

In order to insert a human gene into plasmid, both must

• code for the same gene product.

• be cut by the same restriction enzyme.

• originate from the same type of cell.

• have identical sequences.

In order to insert a human gene into plasmid, both must 

• code for the same gene product.

• be cut by the same restriction enzyme.

• originate from the same type of cell.

• have identical sequences.

Which of the following is a tool used to determine the order of genes on a plasmid?

• Restriction mapping

• Gene cloning

• Polymerase chain reaction

• Gel electrophoresis

What is the primary purpose of plasmid mapping in genetic engineering?

• To identify the plasmid's origin

• To determine the sequence of nucleotides

• To locate restriction enzyme sites

• To measure plasmid size

Which enzyme is commonly used to cut plasmids at specific sites for mapping?

• DNA polymerase

• Ligase

• Restriction enzyme

• Helicase

In plasmid mapping, what does a restriction map show?

• The sequence of amino acids

• The location of restriction sites

• The plasmid's replication origin

• The plasmid's antibiotic resistance

What is the first step in creating a plasmid map?

• Sequencing the plasmid

• Cutting the plasmid with restriction enzymes

• Amplifying the plasmid

• Transforming the plasmid into bacteria