Quiz on L4-5

What is the first step in recombinant DNA technology?

• Ligation

• Cutting of DNA

• Isolation of DNA

• Insertion into vector

Which technique is commonly used for DNA extraction and purification?

• Phenol-chloroform extraction

• Gel electrophoresis

• Blotting

• Polymerase Chain Reaction

What is the purpose of using restriction enzymes in DNA technology?

• To visualize DNA

• To amplify DNA

• To cut DNA at specific sequences

• To ligate DNA fragments

What does PCR stand for?

• Polymerase Chain Reaction

• Protein Chain Reaction

• Polymerase Cell Reaction

• Protein Cell Reaction

Which of the following is NOT a step in the generic rDNA technology process?

• Transformation

• Amplification

• Ligation

• Screening

What is the role of ligase in recombinant DNA technology?

• To transform cells

• To isolate DNA

• To cut DNA

• To join DNA fragments

In the context of PCR, what is the purpose of the denaturation step?

• To separate DNA strands

• To extend primers

• To cool the reaction

• To bind primers

What is the main purpose of gel electrophoresis?

• To amplify DNA

• To extract DNA

• To separate nucleic acid fragments

• To ligate DNA

Which blotting technique is used to detect RNA?

• Eastern blotting

• Western blotting

• Northern blotting

• Southern blotting

What is the primary function of a vector in recombinant DNA technology?

• To visualize DNA

• To amplify DNA

• To carry the gene into the host cell

• To cut DNA