Polymerase Chain Reaction

What is the purpose of the Extension step of PCR?

• To create the primers.

• To extend the time it takes to produce DNA.

• To allow polymerase to create the complementary strands of DNA.

• To allow substrates to copy DNA sequences.

What is the function of a primer in PCR?

• To identify the particular region of DNA to be copied by PCR.

• To copy DNA.

• To create DNA nucleotides.

• To maintain the temperature of the PCR reaction.

The enzyme DNA polymerase used in PCR reaction is isolated from which one of the

following bacteria

• Thermus aquaticus

• Plasmodium vivax

• Both

• None

What process directly follows PCR?

• gel electrophoresis

• DNA sequencing

• cloning

• transformation

Thermus aquaticus is the source of

• Primase enzyme

• Taq Polymerase

• Vent Polymerase

• Both 2 and 3

What does PCR mean?

• polymerase chain restoration

• polymerase chain reaction

• polymerase copying reactions

• polymerase copied repeats

Amplifies or makes numerous copies

• electrophoresis

• wells

• micropipette

• PCR

During PCR, what happens during the Denaturation step?

• DNA strands are separated according to size using gel electrophoresis.

• Primers form base pairs with the single-stranded template DNA.

• DNA polymerase adds new nucleotides to the primer to elongate the new strand.

• Hydrogen bonds between bases are broken using high heat to separate DNA strands.

During which PCR step do primers form complementary base pairs with single-stranded template DNA?

• Denaturation

• Annealing

• Extension

• Synthesis

How is DNA synthesis in PCR and Cycle Sequencing the SAME?

• Both require DNA polymerase.

• Both require primers.

• Both require deoxynucleotides (dNTPs).

• Both require dideoxynucleotides (dNTPs).

• Both generate new DNA products of equal length.