Recombinant DNA

In order to insert a human gene into plasmid, both must

• code for the same gene product.

• originate from the same type of cell.

• be cut by the same restriction enzyme.

• have identical sequences.

Recombinant DNA technology

• is possible only between closely related species

• uses bacteria to make copies of the desired product and splices DNAs together.

• uses bacteria to make copies of the desired product

• uses bacteria to make copies of the desired product, splices DNAs together, and is possible only between closely related species

Tags

NGSS.HS-LS1-1

The following are the uses of

recombinant DNA EXCEPT

• Produce sheep clone

• Produce plants that have bacterial genes to destroy pathogenic insects

• Produce agriculturally important plants and animals

• Produce microbes that secrete enzymes to overcome the problems of oil spoilage in the sea

A technique for making millions of copies of a specific region of DNA.

• DNA ligase

• gel electrophoresis

• restriction enzymes

• polymerase chain reaction

Which is the primary purpose of using restriction enzymes before gel electrophoresis?

• It cuts the DNA strands in a set location for analysis.

• It restricts the number of base pairs that can be tested in a sample

• It makes the testing simpler by moving the strands into the gel faster

• It charges the DNA strands

Which is the primary purpose of using restriction enzymes in gel electrophoresis?

• It allows the strands of DNA to be cut into various lengths for testing

• It restricts the number of base pairs that can be tested in a sample

• It makes the testing simpler by moving the strands into the gel faster

• It charges the DNA strands

A method used to rapidly make multiple copies of a specific segment of DNA.

• PCR (Polymerse Chain Reaction)

• Electrophoresis

• DNA Probe

The first human hormone produced successfully by using DNA recombinant technology is

• human growth hormone

• adenosine deaminase

• insulin

• thyroxin

Recombinant DNA plasmids contain all of the following EXCEPT

• Origin of Replication

• Multiple Cloning Site

• Promoter

• Selectable Marker

• IPTG

​ (a)   are bacterial ​ (b)   that carry genes into bacterial cells. They are created in a lab and contain genes from multiple different sources, thus qualifying them as ​ (c)   e

• Vectors

• plasmids

• recombinant DNA.

• chromosomal DNA

• chromosomes

• bacteria

• antibiotics