MI4.1.2 Safety & Mod 1 Pre-Lab

Students who are allergic to _______ should not participate directly in this lab. If this applies to you, speak with your teacher before the first day of the lab.

• antibiotics

• peanuts

• tomatoes

• bees

• tree nuts

You must wear lab aprons/coats, ________ and ________ when working on this laboratory exercise.

The E. coli we are using in this lab is pathogenic.

• true

• false

Which of the following are important guidelines for working with bacteria in this lab? (Check all that apply.)

• wipe down tabletops with disinfectant before and after lab

• disinfect all materials that contact bacteria before disposing

• wash hands with soap before and water after lab work

• know the protocol BEFORE attempting any tasks

The purpose of Module 1 is to _______ the E. coli bacteria with the pGFP plasmid.

Click on the supernatant

Tags

NGSS.MS-PS1-5

Click on the pellet

You will centrifuge the liquid culture for (a)   , add 200uL of ​ (b)   CaCl2, resuspend the cells by ​ (c)   up and down, and then incubate the cells on ice for ​ (d)   .

• 5 minutes
• cold
• pipetting
• 10 minutes
• hot
• shaking

After the first round of centrifugation, you will add ice-cold ​ (a)   to the pelleted cells. After the second round, you will add ice-cold ​ (b)   .

• calcium chloride
• competent cell solution

Competent cells can be stored for up to ___ hours in the freezer after they have been resuspended in the CSS.

Mathematical Equivalence

OFF