Pglo Lab

Which protein is responsible for allowing the bacteria to grow in the presence of ampicillin?

• GFP
• araC
• Beta Lactamase
• Restriction Enzyme

What is the name of the plasmid we used for lab?

• GFP
• bla
• araC
• pGLO

Which gene is responsible for making the bacteria resistant to ampicillin?

• bla
• pGLO
• araC
• GFP

 What was used to cut the plasmid to enable us to insert the desired gene?

• ligase
• restriction enzymes
• GFP
• pGLO

What was used to create a temperature differential and increase the likelihood of plasmid uptake?

• heat shock

• ice

• arabinose

• CaCl₂

On which plate did bacteria grow and glow?

• LB

• LB/Amp

• LB/Amp/Ara

• All

Tags

NGSS.MS-LS1-5

What is used to select only the bacteria that were transformed?

• ampicillan

• arabinose

• LB

• GFP

Which gene is responsible for the bacteria glowing?

• bla
• araC
• GFP
• pGLO

Tags

NGSS.HS-LS1-1

What is responsible for "turning on" the glow?

• bla

• arabinose

• GFP

• LB

What abbreviation represents the sugar molecule needed to "turn on" the green fluorescent protein gene?

• LB
• AMP
• ARA
• +pGlo