Polymerase Chain Reaction (PCR)

A technique for making millions of copies of a specific region of DNA.

• DNA ligase

• restriction enzymes

• gel electrophoresis

• polymerase chain reaction

At what temperature does the Denature step of PCR occur?

• 95° C

• 50° C

• 72° C

• Any temperature

At what temperature does the Anneal step of PCR occur?

• 95° C

• 50° C

• 72° C

• Any temperature

At what temperature does the Extension step of PCR occur?

• 95°C

• 50-65°C

• 72°C

• Any temperature

What happens during the Anneal step of PCR?

• Primers are created.
• The primers attach to the target DNA region.
• Primers copy the new DNA strand.
• Primers sequence DNA

What is the function of a primer?

• To identify the particular region of DNA to be copied by PCR.
• To copy DNA.
• To create DNA nucleotides.
• To maintain the temperature of the PCR reaction.

What is the purpose of the Extension step of PCR?

• To create the primers.
• To extend the time it takes to produce DNA.
• To allow polymerase to create the complementary strands of DNA.
• To allow substrates to copy DNA sequences.

How many cycles of PCR does it take to produce a double-stranded DNA fragment of the desired length?

• 1
• 2
• 3
• 4

What type of bonds hold the two strands of DNA together?

• Hydrogen bonds
• Covalent bonds
• Dipole-Dipole bonds
• Ionic bonds

What are the base-pairing rules in DNA?

• A-C, G-T
• A-U, G-C
• A-T, G-C
• A-C, G-U